PURIFICATION OF CYTOCHROME C MIXTURE USING SACHEM'S EXPELL™ SP1

Check out the results below with SACHEM’s Expell™ SP1 on Tosoh's TSKgel SP-5PW column.

  Diagram A is the displacement chromatography trace
  Diagram B is the fraction analysis of the displacement chromatography train.
  Finally, Table C is the recovery and purification data from this separation.

Diagram A:  Displacement Chromatography Trace at 280 nm, 264 nm, and 430 nm

results_expellsp1_chart1

Diagram B:  Fraction Analysis Corresponding to the Displacement Chromatography Train Above

results_expellsp1_chart2

Diagram B demonstrates the increased capacity and purity that is attainable using displacement chromatography versus elution chromatography.

Table C:  Resulting Recovery and Purity Data from Fraction Analysis comparing Displacement Chromatography to Elution Chromatography and Self-Displacement Chromatography

comparison_cyto_c_puri