PURIFICATION OF BOVINE β-LACTOGLOBULIN USING SACHEM'S EXPELL™ Q1

Download Free Protocols Check out the results below with SACHEM’s Expell™ Q1 on Tosoh’s TSKgel Super Q column. 

  Diagram A is the displacement chromatography trace. 
  Diagram B is the fraction analysis of the displacement chromatography train. 
  Finally, Table C is the recovery and purification data from this displacement separation.

Diagram A
Displacement Chromatography Trace at 280 nm (protein + displacer) and 312 nm (displacer only)

results_expellq1_chart1


Diagram B:  Fraction Analysis Corresponding to the Displacement Chromatography Train Above



results_expellq1_chart2

Diagram B demonstrates the increased capacity and purity that is attainable using displacement chromatography versus elution chromatography.

Table C:  Resulting Recovery and Purity Data from Fraction Analysis


results_lacto_puri