PURIFICATION OF CYTOCHROME C MIXTURE USING SACHEM'S EXPELL™ SP1 Print E-mail

Displacement chromatography coupled with SACHEM’s Expell™ displacers makes the technique simple enough for every day use.

Check out the results below with SACHEM’s Expell™ SP1 on Tosoh's TSKgel SP-5PW column.

Diagram A is the displacement chromatography trace
Diagram B is the fraction analysis of the displacement chromatography train.
Finally, Table C is the recovery and purification data from this separation.

Diagram A:  Displacement Chromatography Trace at 280 nm, 264 nm, and 430 nm

 

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Diagram B:  Fraction Analysis Corresponding to the Displacement Chromatography Train Above

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Diagram B demonstrates the increased capacity and purity that is attainable using displacement chromatography versus elution chromatography.

Table C:  Resulting Recovery and Purity Data from Fraction Analysis comparing Displacement Chromatography to Elution Chromatography and Self-Displacement Chromatography

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