The results below demonstrate the effectiveness of the Isolis System™ in detecting trace components not identified by elution chromatography analysis alone.

Diagram A is the fraction analysis of the displacement chromatography train, the first step in the Isolis System™.  The displacement chromatography experiment produces purified major component (dark blue) as well as enriched minor component samples that are concentrated in the transition zones (A and B).  These enhanced samples can then be analyzed using any traditional method including elution chromatography.

Table B is the resulting data comparing the number of components and total impurity observed from the Isolis System™ versus traditional elution chromatography of the original sample.

Diagram A:  Displacement Chromatography Fraction Analysis



Diagram A demonstrates how displacement chromatography simultaneously concentrates impurities while removing main component interference.  The impurities are concentrated (10-400X enrichment) in discrete zones (A and B) between main component bands (porcine somatotropin and displacer).

Table B:  Resulting Data
Total Impurity from Isolis System™ utilizing Displacement Chromatography vs. Traditional Elution Chromatography